This report reviews 42 case studies of businesses who have publicly shared their experiences of natural capital assessment. The report finds that business motivations include the desire to identify natural capital risks, the reputational benefits of adopting innovative sustainability approaches, and the synergies between natural capital assessment and larger sustainability approaches and goals. Challenges encountered by these businesses include accessing sufficient high-resolution data, obtaining support for natural capital assessment as decision-making rather than just a reporting tool, and the uncertainty associated with assessment methods which are still maturing. In addition, these case studies suggest that, going forward, key research needs include improving the datasets and methods available to support natural capital assessment and investigating the extent to which monetary assessment translates into positive outcomes for natural capital.